Fig. 2.

In vitro activation of DCs by different formulations. a and b show the amount of IL-12 and TNF-alpha secreted by BMDCs after 48 h of incubation with PBS, MIP, BCG, BAP, MEAP and BEAP. IFN-gamma secretion and proliferation of T cells in allogenic MLR when co-cultured with BMDCs pre-stimulated with different formulations are shown in (c and d). Similar allogenic MLR was performed with lung DCs and results are shown in (e and f). All the panels indicate that alginate coated mycobacterium leads to higher activation of DCs compared to the suspension of bacteria