Table 2.
Alignment of the NH2-terminal amino-acid sequence of the purified SPPS from Pleurotus sajor-caju strain CTM10057 with the sequences of other fungal proteases
| Enzyme | Origin | NH2-terminal amino-acid a,b | Identity (%) |
|---|---|---|---|
| SPPS (this work) | Pleurotus sajor-caju strain CTM10057 | GPEDPALPPDSESTHVITGVEKLHAQ | – |
| PoSl | Pleurotus ostreatus strain ATCC® MYA-2306™ | GP D DPALPPD | 90 |
| PA_PoS1 | Schizopora paradoxa strain KUC8140 | GP T DPA I PPDSESTHV L TGV | 85 |
| VPR | Phlebia centrifuga strain FBCC195 | DDPAI PPD T ESTHV L TGV D KLHAQ | 79 |
| PcSl | Phanerochaete chrysosporium strain S1 | G KS DPA V P A D T ESTH | 75 |
| PR1C | Metarhizium anisopliae strain ARSEF 549 | LSSR A DDGA D IF S P HV MTQV KL | 64 |
a Amino-acid sequences for comparison were obtained using the program BLASTP (NCBI, NIH, USA) database with default parameters
b Residues not identical with SPPS protease from Pleurotus sajor-caju strain CTM10057 are indicated in black box