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. 2019 Jun 16;2019:7283683. doi: 10.1155/2019/7283683

Figure 5.

Figure 5

The Akt inhibitor LY294002 and Nrf2 siRNA attenuate the antioxidative and cell protective effects of TRPV4 antagonist HC in H9C2 cells exposed to H/R. Images of ROS generation (a) were stained with DCFHDA and generated by fluorescence microscopy. Quantitative analysis of ROS generation (b) was conducted using an Enspire multimode plate reader. MDA levels were measured by the spectrophotometric method (c). Cell viability was detected by a CCK-8 reduction assay (d). LDH release was measured by LDH assay kits (e). Scale bar: 50 μm. Values are presented as the mean ± SEM; n = 6-10/group. We used a one-way ANOVA followed by a Bonferroni test. # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. H/R; & p < 0.05 and &&& p < 0.001 vs. H/R+HC; and $ p < 0.05 and $$$ p < 0.001 vs. H/R+Con siRNA.