Fig. 3.

Increased expression of miR-148b-3p in human BM-MSCs was potentially mediated by LV enhanced osteogenic differentiation. a After 21 days mineral nodules depositions were detected with ARS staining. b The stained cells were eluted using 10% acetic acid and the absorbance of extracted stain was measured at 405 nm to quantify the mineralization level. c ALP production was evaluated with BCIP/NBT kit as dark purples deposits. d After 14 days ALP activity was quantified with pNPP substrate system.****P < 0.0001