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. 2019 Jul 1;38:283. doi: 10.1186/s13046-019-1279-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Cdx1 serves as a transcriptional factor of MUC17. a Luciferase assays using AGS cells transfected with one of three MUC17 promoter fragments with differently sized deletions (FA-FC). b The luciferase activity of the FA fragment in AGS cells transfected with a shRNA targeting CDX1. c The luciferase activity of FA and FB in MKN45 cells transfected with CDX1. d Upper, ChIP assay revealed that interacted CDX1 with the promoter region of MUC17. Lower, ChIP assay indicated knocked-down of CDX1 did not impact the transcriptional state of chromatin by using the antibody against H4 acetylated K5. e Luciferase assay using the FA fragment and its mutant derivatives transfected into AGS cells (left) and MKN45 cells overexpressing CDX1 (right). f EMSA assay confirmed that CDX1 binding to the second motif of the MUC17 promoter. g The expression of MUC17 and CDX1 mRNA detected by qPCR in GC cell lines (left). The correlation between CDX1 and MUC17 expression in GC cell lines (right). Error bars represent ± SD of three experiments (*, P < 0.05; **, P < 0.001)