Table 6.
Selected genes and primers used for gene expression analysis of intestinal tissue
| Gene name | Entrez gene ID | Primer sequence | Tm | Product size (bp) | Gene Function |
|---|---|---|---|---|---|
| LYN | 100,302,103 |
5′-ACGGAGAGTGGTGGAAAGC-3′ 5′-GTGCACGGGGTCATAGTCT-3’ |
59.6 59.1 |
482 | B cell signaling + activation |
| GRB2 | 101,109,893 |
5’-ACTGCTGCTCCTGTTCTTCC-3′ 5′-AAACGCAGAACACAGAAGCG-3’ |
60.0 59.7 |
442 | B cell signal transduction + communication |
| EGR1 | 443,547 |
5’-CCCCGACTATCTGTTTCCACA-3′ 5′-ATGCGGCTGGGTTTGATGA-3’ |
59.5 60.0 |
340 | B cell maturation + activating factor signaling |
| JUN | 443,219 |
5’-CAAGTGCCGGAAAAGGAAGC-3′ 5′-ACAGTCTCGCCTCAAAACGT-3’ |
59.5 59.8 |
314 | Transcription regulator |
| NFIL3 | 100,217,409 |
5’-CACTGTGAGCGCCTTTGTG-3′ 5′-GGGCCCTCCTGTGAATGTT-3’ |
59.7 59.6 |
262 | B Cell survival |
| LCK | 100,216,439 |
5’-CCCAGCTTCTCCACTGCAA-3′ 5′-ACGGAGCTGTTCACCCTTC-3’ |
59.9 59.6 |
270 | B cell activation |
| CD81 | 100,147,790 |
5’-CTGGGCACGTTCTTCACCT-3′ 5′-GCTGCAAGGCCTGGTCATA-3’ |
59.3 60.0 |
479 | B cell development, activation, growth and motility |
| CD84 | 101,102,779 |
5’-CGTGGAACCCTGTCAGCAA-3′ 5′-AGCACAGAAAGCACAGCCA-3’ |
60.2 60.2 |
412 | Found on memory B cells |
| CD40L | 767,628 |
5’-AGCTGGCCGTGAAAAGACA-3′ 5′-AACACCGAAGCACCCGATT-3’ |
59.9 59.9 |
489 | Initiation and maintenance of humoral immune response |
| BAFF (TNFSF13B) | 101,104,901 |
5’-TTGCAGACAGTGACACGCC-3′ 5′-AGGTGTCCCATGGCAAAGG-3’ |
60.9 59.9 |
426 | B Cell survival |
| MIF | 780,466 |
5’-GCAAGCCGGCACAGTACAT-3′ 5′-ATGTAGATCCTGTCCGGGCT-3’ |
61.0 60.1 |
305 | Survival of mature B cells + NF-kB signalling cascade |
| Reference gene | |||||
| Ovine GAPDH | 443,005 |
5’-AGAAACCTGCCAAGTATGATG-3′ 5′-CCTAGAATGCCCTTGAGAGG-3’ |
60.5 62.6 |
76 | |