Table 10.

Calculated K_sec and% OK_sec (from the reference K_sec at 60 °C) from elution volumes obtained for PS standards (Mp 282,000; 126,000; 51,200; 19,500; 7350; 2780; 580) on cyano column with a variety of 70/30 THF/solvent j mobile phases. Instrumentation and other conditions are as in Table 4.

Column ID	Mobile Phase	PS Mp	Temperature
			10 °C		20 °C		30 °C		40 °C		50 °C		60 °C
			Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec Reference
Cyano	70/30 (v/v) THF/MeOH	282000	0.055	−6.8	0.057	−3.4	0.056	−5.1	0.057	−3.4	0.058	−1.7	0.059
		126000	0.161	−1.8	0.164	0.0	0.161	−1.8	0.163	−0.6	0.163	−0.6	0.164
		51200	0.356	−1.1	0.360	0.0	0.356	−1.1	0.358	−0.6	0.359	−0.3	0.360
		19500	0.571	−0.7	0.577	0.3	0.570	−0.9	0.572	−0.5	0.573	−0.3	0.575
		7350	0.742	−0.4	0.749	0.5	0.740	−0.7	0.743	−0.3	0.743	−0.3	0.745
		2780	0.847	−0.4	0.854	0.5	0.845	−0.6	0.847	−0.4	0.848	−0.2	0.850
		580	0.940	−0.2	0.948	0.6	0.938	−0.4	0.940	−0.2	0.941	−0.1	0.942
	70/30 (v/v) THF/ACN	282000	0.055	−1.8	0.055	−1.8	0.055	−1.8	0.056	0.0	0.055	−1.8	0.056
		126000	0.155	0.0	0.156	0.6	0.156	0.6	0.156	0.6	0.155	0.0	0.155
		51200	0.350	0.6	0.350	0.6	0.350	0.6	0.350	0.6	0.349	0.3	0.348
		19500	0.568	0.2	0.569	0.4	0.568	0.2	0.568	0.2	0.568	0.2	0.567
		7350	0.744	0.0	0.745	0.1	0.743	−0.1	0.744	0.0	0.744	0.0	0.744
		2780	0.852	0.0	0.853	0.1	0.851	−0.1	0.852	0.0	0.853	0.1	0.852
		580	0.949	0.0	0.950	0.1	0.948	−0.1	0.949	0.0	0.950	0.1	0.949
	70/30 (v/v) THF/EtOH	282000	0.053	−7.0	0.055	−3.5	0.055	−3.5	0.056	−1.8	0.056	−1.8	0.057
		126000	0.158	−1.9	0.159	−1.2	0.159	−1.2	0.160	−0.6	0.160	−0.6	0.161
		51200	0.354	−1.1	0.355	−0.8	0.355	−0.8	0.357	−0.3	0.356	−0.6	0.358
		19500	0.572	−0.7	0.574	−0.3	0.574	−0.3	0.575	−0.2	0.574	−0.3	0.576
		7350	0.746	−0.7	0.748	−0.4	0.748	−0.4	0.750	0.1	0.749	−0.3	0.751
		2780	0.853	−0.5	0.855	−0.2	0.855	−0.2	0.857	0.0	0.856	−0.1	0.857
		580	0.947	−0.5	0.949	−0.3	0.949	−0.3	0.952	0.0	0.950	−0.2	0.952
	70/30 (v/v) THF/DMF	282000	0.021	−48.8	0.018	−56.1	0.022	−46.3	0.037	−9.8	0.032	−22.0	0.041
		126000	0.135	−5.6	0.139	−2.8	0.141	−1.4	0.142	−0.7	0.141	−1.4	0.143
		51200	0.325	−2.7	0.329	−1.5	0.331	−0.9	0.332	−0.6	0.331	−0.9	0.334
		19500	0.553	−1.4	0.556	−0.9	0.559	−0.4	0.559	−0.4	0.558	−0.5	0.561
		7350	0.741	−1.1	0.743	−0.8	0.746	0.4	0.746	0.4	0.746	0.4	0.749
		2780	0.857	−0.9	0.859	−0.7	0.862	−0.3	0.863	−0.2	0.862	−0.3	0.865
		580	0.960	−0.9	0.962	−0.7	0.966	−0.3	0.967	−0.2	0.966	−0.3	0.969
	70/30 (v/v) THF/IPA	282000	0.053	−5.4	0.053	−5.4	0.055	−1.8	0.055	−1.8	0.056	0.0	0.056
		126000	0.160	0.0	0.159	−0.6	0.160	0.0	0.159	−0.6	0.160	0.0	0.160
		51200	0.358	0.3	0.357	0.0	0.358	0.3	0.357	0.0	0.357	0.0	0.357
		19500	0.578	0.2	0.578	0.2	0.577	0.0	0.577	0.0	0.577	0.0	0.577
		7350	0.753	0.1	0.753	0.1	0.752	0.0	0.752	0.0	0.752	0.0	0.752
		2780	0.860	0.2	0.860	0.2	0.858	0.0	0.859	0.1	0.859	0.1	0.858
		580	0.951	−0.1	0.952	0.0	0.950	−0.2	0.952	0.0	0.952	0.0	0.952
	70/30 (v/v) THF/chloroform	282000	0.026	−33.3	0.020	−48.7	0.036	−7.7	0.038	−2.6	0.039	0.0	0.039
		126000	0.142	−1.4	0.144	0.0	0.144	0.0	0.144	0.0	0.145	0.7	0.144
		51200	0.334	−0.6	0.336	0.0	0.335	−0.3	0.335	−0.3	0.336	0.0	0.336
		19500	0.558	−0.4	0.560	0.0	0.559	−0.2	0.559	−0.2	0.560	0.0	0.560
		7350	0.740	−0.3	0.742	0.0	0.741	−0.1	0.741	−0.1	0.742	0.0	0.742
		2780	0.852	−0.1	0.853	0.0	0.853	0.0	0.852	−0.1	0.853	0.0	0.853
		580	0.947	−0.1	0.949	0.1	0.949	0.1	0.948	0.0	0.948	0.0	0.948
	100% THF	282000	0.042	−8.7	0.044	−4.3	0.046	0.0	0.044	−4.3	0.045	−2.2	0.046
		126000	0.135	−2.9	0.136	−2.2	0.138	0.7	0.137	−1.4	0.138	−0.7	0.139
		51200	0.319	−1.5	0.321	−0.9	0.323	−0.3	0.322	−0.6	0.323	−0.3	0.324
		19500	0.543	−0.7	0.544	−0.5	0.546	−0.2	0.544	−0.5	0.546	−0.2	0.547
		7350	0.728	−0.4	0.730	−0.1	0.731	0.0	0.729	−0.3	0.731	0.0	0.731
		2780	0.843	−0.2	0.845	0.0	0.846	0.1	0.843	−0.2	0.845	0.0	0.845
		580	0.945	0.0	0.947	0.2	0.948	0.3	0.945	0.0	0.946	0.1	0.945