. Author manuscript; available in PMC: 2019 Jul 2.

Published in final edited form as: J Chromatogr A. 2017 Nov 24;1531:83–103. doi: 10.1016/j.chroma.2017.11.027

Table 11.

Calculated K_sec and% ΔK_sec (from the reference K_sec at 60 °C) from elution volumes obtained for PMMA standards (Mp 46,890; 20,310; 7360; 3070) on cyano column with a variety of 70/30 THF/solvent j mobile phases. Instrumentation and other conditions are as in Table 5.

Column ID	Mobile Phase	PMMA Mp	Temperature
			10 °C		20 °C		30 °C		40 °C		50 °C		60 °C
			K_sec	% ∆K_sec	K_sec	% ∆K_sec	K_sec	% ∆K_sec	K_sec	% ∆K_sec	K_sec	% ∆K_sec	K_{sec Reference}
Cyano	70/30 THF/MeOH	46890	0.414	6.4	0.412	5.9	0.402	3.3	0.396	1.8	0.392	0.8	0.389
		20310	0.627	3.0	0.627	3.0	0.617	1.3	0.613	0.7	0.610	0.2	0.609
		7360	0.799	1.4	0.802	1.8	0.791	0.4	0.789	0.1	0.788	0.0	0.788
		3070	0.876	0.1	0.883	0.9	0.874	−0.1	0.873	−0.2	0.874	−0.1	0.875
	70/30 THF/ACN	46890	0.378	3.6	0.376	3.0	0.374	2.5	0.370	1.4	0.367	0.5	0.365
		20310	0.597	1.9	0.595	1.5	0.594	1.4	0.590	0.7	0.588	0.3	0.586
		7360	0.775	0.4	0.776	0.5	0.776	0.5	0.772	0.0	0.772	0.0	0.772
		3070	0.867	−0.1	0.868	0.0	0.870	0.2	0.867	−0.1	0.867	−0.1	0.868
	70/30 THF/EtOH	46890	0.437	8.7	0.428	6.5	0.421	4.7	0.414	3.0	0.407	1.2	0.402
		20310	0.647	4.2	0.641	3.2	0.635	2.3	0.630	1.4	0.625	0.6	0.621
		7360	0.814	2.0	0.809	1.4	0.805	0.9	0.803	0.6	0.800	0.3	0.798
		3070	0.889	0.3	0.888	0.2	0.887	0.1	0.887	0.1	0.886	0.0	0.886
	70/30 THF/IPA	46890	0.460	11.1	0.447	8.0	0.438	5.8	0.428	3.4	0.420	1.4	0.414
		20310	0.669	5.7	0.659	4.1	0.651	2.8	0.644	1.7	0.637	0.6	0.633
		7360	0.844	4.5	0.826	2.2	0.820	1.5	0.814	0.7	0.810	0.2	0.808
		3070	0.902	1.1	0.899	0.8	0.896	0.4	0.894	0.2	0.892	0.0	0.892
	100% THF	46890	0.396	3.4	0.395	3.1	0.394	2.9	0.389	1.6	0.386	0.8	0.383
		20310	0.614	1.8	0.613	1.7	0.613	1.7	0.607	0.7	0.606	0.5	0.603
		7360	0.791	0.5	0.791	0.5	0.793	0.8	0.788	0.1	0.788	0.1	0.787
		3070	0.882	0.2	0.882	0.2	0.884	0.5	0.880	0.0	0.881	0.1	0.880