Table 5.

Calculated K_sec and% ΔK_sec (from the reference Ksec at 60 °C) from elution volumes obtained for PMMA standards (Mp 46,890; 20,310; 7360; 3070) on C₁₈ column with a variety of 70/30 THF/solvent j mobile phases. Instrumentation: Agilent 1100 with a variable wavelength detector set to 230 nm. Other conditions: injection volume = 1.0 µL; flow rate = 0.30 mL min⁻¹; column temperature varied from 10 °C to 60 °C.

Column ID	Mobile Phase	PMMA Mp	Temperature
			10 °C		20 °C		30 °C		40 °C		50 °C		60 °C
			Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec Reference
C18	70/30 (v/v) THF/MeOH	46890	0.354	6.0	0.349	4.5	0.345	3.3	0.342	2.4	0.337	0.9	0.334
		20310	0.544	2.3	0.541	1.7	0.539	1.3	0.537	0.9	0.533	0.2	0.532
		7360	0.698	0.0	0.698	0.0	0.698	0.0	0.698	0.0	0.697	−0.1	0.698
		3070	0.783	−1.0	0.785	−0.8	0.787	−0.5	0.788	−0.4	0.789	−0.3	0.791
	70/30 (v/v) TH F/ACN	46890	0.329	2.2	0.328	1.9	0.326	1.2	0.325	0.9	0.323	0.3	0.322
		20310	0.526	0.4	0.526	0.4	0.525	0.2	0.525	0.2	0.524	0.0	0.524
		7360	0.688	−1.1	0.690	−0.9	0.691	−0.7	0.693	−0.4	0.694	−0.3	0.696
		3070	0.779	−1.6	0.782	−1.3	0.785	−0.9	0.787	−0.6	0.790	−0.3	0.792
	70/30 (v/v) THF/EtOH	46890	0.362	6.2	0.356	4.4	0.352	3.2	0.348	2.1	0.343	0.6	0.341
		20310	0.549	2.2	0.545	1.5	0.543	1.1	0.541	0.7	0.538	0.2	0.537
		7360	0.701	−0.1	0.700	−0.3	0.701	−0.1	0.701	−0.1	0.701	−0.1	0.702
		3070	0.786	−1.1	0.787	−1.0	0.789	−0.8	0.791	0.5	0.793	−0.3	0.795
	70/30 (v/v) THF/IPA	46890	0.354	6.0	0.349	4.5	0.348	4.2	0.341	2.1	0.338	1.2	0.334
		20310	0.541	2.1	0.537	1.3	0.535	0.9	0.533	0.6	0.533	0.6	0.530
		7360	0.692	−0.4	0.692	−0.4	0.690	−0.7	0.693	−0.3	0.695	0.0	0.695
		3070	0.777	−1.4	0.779	−1.1	0.777	−1.4	0.784	−0.5	0.787	−0.1	0788
	100% THF	46890	0.342	3.3	0.340	2.7	0.339	2.4	0.337	1.8	0.333	0.6	0.331
		20310	0.538	1.1	0.537	0.9	0.536	0.8	0.535	0.6	0.532	0.0	0.532
		7360	0.704	−0.1	0.705	0.0	0.705	0.0	0.706	0.1	0.705	0.0	0.705
		3070	0.797	−0.7	0.799	−0.5	0.800	−0.4	0.802	−0.1	0.802	−0.1	0.803