Table 6.

Calculated K_sec and% ΔK_sec (from the reference Ksec at 60 °C) from elution volumes obtained for PS standards (Mp 282,000; 126,000; 51,200; 19,500; 7350; 2780; 580) on C₄ column with a variety of 70/30 THF/solvent j mobile phases. Instrumentation and other conditions are as in Table 4.

Column ID	Mobile Phase	PMMA Mp	Temperature
			10 °C		20 °C		30 °C		40 °C		50 °C		60 °C
			Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec Reference
C4	70/30 (v/v) THF/MeOH	282000	0.068	−1.4	0.068	−1.4	0.069	0.0	0.068	−1.4	0.069	0.0	0.069
		126000	0.191	0.0	0.191	0.0	0.191	0.0	0.191	0.0	0.191	0.0	0.191
		51200	0.419	0.2	0.418	0.0	0.418	0.0	0.418	0.0	0.418	0.0	0.418
		19500	0.644	0.2	0.644	0.2	0.643	0.0	0.644	0.2	0.644	0.2	0.643
		7350	0.812	0.2	0.811	0.1	0.811	0.1	0.812	0.2	0.811	0.1	0.810
		2780	0.910	0.2	0.909	0.1	0.908	0.0	0.909	0.1	0.908	0.0	0.908
		580	0.990	0.2	0.989	0.1	0.988	0.0	0.989	0.1	0.988	0.0	0.988
	70/30 (v/v) THF/ACN	282000	0.067	0.0	0.067	0.0	0.067	0.0	0.067	0.0	0.067	0.0	0.067
		126000	0.191	2.1	0.190	1.6	0.189	1.1	0.189	1.1	0.188	0.5	0.187
		51200	0.427	1.7	0.426	1.4	0.423	0.7	0.423	0.7	0.421	0.2	0.420
		19500	0.659	1.1	0.658	0.9	0.655	0.5	0.656	0.6	0.654	0.3	0.652
		7350	0.825	0.5	0.825	0.5	0.824	0.4	0.824	0.4	0.823	0.2	0.821
		2780	0.919	0.2	0.919	0.2	0.918	0.1	0.919	0.2	0.919	0.2	0.917
		580	0.990	−0.1	0.991	0.0	0.990	−0.1	0.992	0.1	0.992	0.1	0.991
	70/30 (v/v) THF/EtOH	282000	0.054	−6.9	0.055	−5.2	0.057	−1.7	0.057	−1.7	0.057	−1.7	0.058
		126000	0.161	−1.8	0.162	−1.2	0.163	−0.6	0.163	−0.6	0.163	−0.6	0.164
		51200	0.369	−1.3	0.370	−1.1	0.372	−0.5	0.372	−0.5	0.373	−0.3	0.374
		19500	0.590	−1.2	0.591	−1.0	0.594	−0.5	0.594	−0.5	0.596	−0.2	0.597
		7350	0.763	−1.0	0.765	−0.8	0.767	−0.5	0.768	−0.4	0.770	−0.1	0.771
		2780	0.869	−0.9	0.871	−0.7	0.873	−0.5	0.874	−0.3	0.876	−0.1	0.877
		580	0.966	−0.6	0.968	−0.4	0.969	−0.3	0.970	−0.2	0.972	0.0	0.972
	70/30 (v/v) THF/EtOH	282000	0.049	−5.8	0.050	−3.8	0.051	−1.9	0.051	−1.9	0.052	0.0	0.052
		126000	0.148	0.0	0.147	−0.7	0.149	0.7	0.148	0.0	0.148	0.0	0.148
		51200	0.353	0.0	0.352	−0.3	0.354	0.3	0.352	−0.3	0.353	0.0	0.353
		19500	0.583	0.2	0.583	0.2	0.584	0.3	0.582	0.0	0.582	0.0	0.582
		7350	0.765	0.4	0.764	0.3	0.764	0.3	0.763	0.1	0.763	0.1	0.762
		2780	0.873	0.3	0.872	0.2	0.872	0.2	0.871	0.1	0.871	0.1	0.870
		580	0.962	0.2	0.961	0.1	0.960	0.0	0.961	0.1	0.960	0.0	0.960
	70/30 (v/v) THF/IPA	282000	0.057	−5.0	0.058	−3.3	0.059	−1.7	0.059	−1.7	0.060	0.0	0.060
		126000	0.167	−1.2	0.168	−0.6	0.167	−1.2	0.168	−0.6	0.169	0.0	0.169
		51200	0.378	−1.0	0.380	−0.5	0.380	−0.5	0.381	−0.3	0.382	0.0	0.382
		19500	0.600	−1.0	0.602	−0.7	0.602	−0.7	0.603	−0.5	0.604	−0.3	0.606
		7350	0.771	−0.8	0.773	−0.5	0.773	−0.5	0.775	−0.3	0.776	−0.1	0.777
		2780	0.875	−0.7	0.876	−0.6	0.877	−0.5	0.878	−0.3	0.880	−0.1	0.881
		580	0.967	−0.5	0.968	−0.4	0.968	−0.4	0.969	−0.3	0.970	−0.2	0.972
	70/30 (v/v) THF/chloroform	282000	0.048	−4.0	0.048	−4.0	0.049	−2.0	0.049	−2.0	0.050	0.0	0.050
		126000	0.144	−0.7	0.144	−0.7	0.144	−0.7	0.145	0.0	0.145	0.0	0.145
		51200	0.343	−0.6	0.344	−0.3	0.344	−0.3	0.344	−0.3	0.344	−0.3	0.345
		19500	0.567	−0.5	0.569	−0.2	0.569	−0.2	0.569	−0.2	0.570	0.0	0.570
		7350	0.747	−0.4	0.749	−0.1	0.749	−0.1	0.749	−0.1	0.750	0.0	0.750
		2780	0.858	−0.3	0.860	−0.1	0.860	−0.1	0.860	−0.1	0.861	0.0	0.861
		580	0.960	−0.2	0.962	0.0	0.962	0.0	0.962	0.0	0.962	0.0	0.962
	100% THF	282000	0.043	−8.5	0.044	−6.4	0.044	−6.4	0.046	−2.1	0.046	−2.1	0.047
		126000	0.134	−3.6	0.135	−2.9	0.134	−3.6	0.137	−1.4	0.138	−0.7	0.139
		51200	0.329	−2.1	0.331	−1.5	0.328	−2.4	0.333	−0.9	0.333	−0.9	0.336
		19500	0.556	−1.4	0.559	−0.9	0.554	−1.8	0.561	−0.5	0.561	−0.5	0.564
		7350	0.742	−0.9	0.744	−0.7	0.737	−1.6	0.745	−0.5	0.746	−0.4	0.749
		2780	0.856	−0.8	0.859	−0.5	0.850	−1.5	0.859	−0.5	0.860	−0.3	0.863
		580	0.961	−0.5	0.963	−0.3	0.953	−1.3	0.963	−0.3	0.963	−0.3	0.966