Table 8.

Calculated K_sec and% ΔK_sec (from the reference K_sec at 60 °C) from elution volumes obtained for PS standards (Mp 282,000; 126,000; 51,200; 19,500; 7350; 2780; 580) on phenyl column with a variety of 70/30 THF/solvent j mobile phases. Instrumentation and other conditions are as in Table 4.

Column ID	Mobile Phase	PS Mp	Temperature
			10 °C		20 °C		30 °C		40 °C		50 °C		60 °C
			Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec	% ∆Ksec	Ksec Reference
Phenyl	70/30 (v/v) THF/MeOH	282000	0.088	−2.2	0.087	−3.3	0.089	−1.1	0.089	−1.1	0.090	0.0	0.090
		126000	0.243	−1.2	0.242	−1.6	0.244	−0.8	0.244	−0.8	0.245	−0.4	0.246
		51200	0.498	−0.8	0.499	−0.6	0.500	−0.4	0.502	0.0	0.502	0.0	0.502
		19500	0.718	−0.6	0.718	−0.6	0.719	−0.4	0.722	0.0	0.721	−0.1	0.722
		7350	0.861	−0.3	0.862	−0.2	0.862	−0.2	0.865	0.1	0.864	0.0	0.864
		2780	0.934	−0.3	0.935	−0.2	0.936	−0.1	0.938	0.1	0.937	0.0	0.937
		580	0.984	−0.2	0.985	−0.1	0.985	−0.1	0.987	0.1	0.986	0.0	0.986
	70/30 (v/v) THF/ACN	282000	0.072	−2.7	0.073	−1.4	0.073	−1.4	0.073	−1.4	0.073	−1.4	0.074
		126000	0.206	−1.0	0.206	−1.0	0.206	−1.0	0.207	−0.5	0.207	−0.5	0.208
		51200	0.449	−0.9	0.450	−0.7	0.449	−0.9	0.451	−0.4	0.452	−0.2	0.453
		19500	0.675	−0.7	0.676	−0.6	0.676	−0.6	0.679	−0.1	0.678	−0.3	0.680
		7350	0.832	−0.5	0.833	−0.4	0.833	−0.4	0.835	−0.1	0.836	0.0	0.836
		2780	0.917	−0.3	0.918	−0.2	0.918	−0.2	0.920	0.0	0.920	0.0	0.920
		580	0.979	−0.3	0.980	−0.2	0.980	−0.2	0.983	0.1	0.982	0.0	0.982
	70/30 (v/v) THF/EtOH	282000	0.070	−2.8	0.071	−1.4	0.070	−2.8	0.071	−1.4	0.071	−1.4	0.072
		126000	0.201	−0.5	0.201	−0.5	0.201	−0.5	0.201	−0.5	0.201	−0.5	0.202
		51200	0.440	−0.2	0.440	−0.2	0.440	−0.2	0.440	−0.2	0.441	0.0	0.441
		19500	0.666	−0.1	0.666	−0.1	0.667	0.0	0.667	0.0	0.668	0.1	0.667
		7350	0.825	−0.1	0.825	−0.1	0.826	0.0	0.827	0.1	0.827	0.1	0.826
		2780	0.913	0.0	0.913	0.0	0.913	0.0	0.914	0.1	0.915	0.2	0.913
		580	0.978	−0.1	0.978	−0.1	0.979	0.0	0.980	0.1	0.981	0.2	0.979
	70/30 (v/v) THF/DMF	282000	0.052	−5.5	0.052	−5.5	0.053	−3.6	0.052	−5.5	0.054	−1.8	0.055
		126000	0.156	−1.3	0.156	−1.3	0.158	0.0	0.155	−1.9	0.157	−0.6	0.158
		51200	0.370	−0.8	0.370	−0.8	0.374	−0.3	0.369	−1.1	0.372	−0.3	0.373
		19500	0.600	−0.3	0.600	−0.3	0.603	0.2	0.599	−0.5	0.602	0.0	0.602
		7350	0.775	−0.3	0.775	−0.3	0.777	0.0	0.774	−0.4	0.776	−0.1	0.777
		2780	0.877	−0.1	0.877	−0.1	0.878	0.0	0.876	−0.2	0.878	0.0	0.878
		580	0.962	0.0	0.962	0.0	0.962	0.0	0.961	−0.1	0.962	0.0	0.962
	70/30 (v/v) THF/IPA	282000	0.074	−1.3	0.074	−1.3	0.074	−1.3	0.074	−1.3	0.075	0.0	0.075
		126000	0.209	−0.5	0.209	−0.5	0.209	−0.5	0.209	−0.5	0.210	0.0	0.210
		51200	0.452	−0.2	0.452	−0.2	0.452	−0.2	0.453	0.0	0.453	0.0	0.453
		19500	0.677	−0.4	0.679	−0.1	0.678	−0.3	0.679	−0.1	0.680	0.0	0.680
		7350	0.834	−0.4	0.835	−0.2	0.835	−0.2	0.836	−0.1	0.836	−0.1	0.837
		2780	0.919	−0.2	0.920	−0.1	0.920	−0.1	0.921	0.0	0.921	0.0	0.921
		580	0.981	−0.2	0.982	−0.1	0.983	0.0	0.984	0.1	0.984	0.1	0.983
	70/30 (v/v) THF/chloroform	282000	0.053	−5.4	0.053	−5.4	0.054	−3.6	0.055	−1.8	0.056	0.0	0.056
		126000	0.161	−1.2	0.161	−1.2	0.161	−1.2	0.162	−0.6	0.163	0.0	0.163
		51200	0.378	−0.8	0.379	−0.5	0.378	−0.8	0.380	−0.3	0.381	0.0	0.381
		19500	0.606	−0.7	0.607	−0.5	0.607	−0.5	0.608	−0.3	0.609	−0.2	0.610
		7350	0.778	−0.5	0.780	−0.3	0.779	−0.4	0.781	−0.1	0.781	−0.1	0.782
		2780	0.878	−0.6	0.881	−0.2	0.880	−0.3	0.882	−0.1	0.882	−0.1	0.883
		580	0.963	−0.3	0.965	−0.1	0.964	−0.2	0.966	0.0	0.966	0.0	0.966
	100% THF	282000	0.053	−3.6	0.053	−3.6	0.053	−3.6	0.054	−1.8	0.055	0.0	0.055
		126000	0.152	−2.6	0.152	−2.6	0.153	−1.9	0.154	−1.3	0.155	−0.6	0.156
		51200	0.359	−1.6	0.360	−1.4	0.361	−1.1	0.362	−0.8	0.364	−0.3	0.365
		19500	0.583	−1.0	0.584	−0.8	0.585	−0.7	0.586	−0.5	0.588	−0.2	0.589
		7350	0.761	−0.5	0.761	−0.5	0.761	−0.5	0.762	−0.4	0.764	−0.1	0.765
		2780	0.865	−0.3	0.865	−0.3	0.866	−0.2	0.866	−0.2	0.867	−0.1	0.868
		580	0.955	0.0	0.954	−0.1	0.954	−0.1	0.954	−0.1	0.955	0.0	0.955