. Author manuscript; available in PMC: 2019 Jul 2.

Published in final edited form as: J Chromatogr A. 2017 Nov 24;1531:83–103. doi: 10.1016/j.chroma.2017.11.027

Table 9.

Calculated K_sec and% OK_sec (from the reference K_sec at 60 °C) from elution volumes obtained for PMMA standards (Mp 46,890; 20,310; 7360; 3070) on phenyl column with a variety of 70/30 THF/solvent j mobile phases. Instrumentation and other conditions are as in Table 5.

Column ID	Mobile Phase	PMMA Mp	Temperature
			10 °C		20 °C		30 °C		40 °C		50 °C		60 °C
			K_sec	% ∆K_sec	K_sec	% ∆K_sec	K_sec	% ∆K_sec	K_sec	% ∆K_sec	K_sec	% ∆K_sec	K_{sec Reference}
Phenyl	70/30 (v/v) THF/MeOH	46890	0.466	7.1	0.457	5.1	0.451	3.7	0.442	1.6	0.440	1.1	0.435
		20310	0.676	3.4	0.670	2.4	0.665	1.7	0.659	0.8	0.657	0.5	0.654
		7360	0.824	1.1	0.821	0.7	0.819	0.5	0.816	0.1	0.816	0.1	0.815
		3070	0.898	0.3	0.897	0.2	0.896	0.1	0.894	−0.1	0.407	0.1	0.895
	70/30 (v/v) THF/ACN	46890	0.417	3.0	0.414	2.2	0.413	2.0	0.409	1.0	0.407	0.5	0.405
		20310	0.634	1.3	0.631	0.8	0.632	1.0	0.629	0.5	0.627	0.2	0.626
		7360	0.795	0.0	0.794	−0.1	0.797	0.3	0.795	0.0	0.795	0.0	0.795
		3070	0.879	−0.5	0.879	−0.5	0.883	0.0	0.882	−0.1	0.882	−0.1	0.883
	70/30 (v/v) THF/EtOH	46890	0.486	9.5	0.476	7.2	0.466	5.0	0.458	3.2	0.450	1.4	0.444
		20310	0.687	4.6	0.680	3.5	0.673	2.4	0.668	1.7	0.662	0.8	0.657
		7360	0.830	1.7	0.827	1.3	0.823	0.9	0.822	0.7	0.819	0.4	0.816
		3070	0.902	0.6	0.901	0.4	0.900	0.3	0.900	0.3	0.899	0.2	0.897
	70/30 (v/v) THF/IPA	46890	0.479	8.9	0.470	6.8	0.460	4.5	0.453	3.0	0.446	1.4	0.440
		20310	0.684	4.1	0.677	3.0	0.671	2.1	0.665	1.2	0.660	0.5	0.657
		7360	0.831	1.6	0.828	1.2	0.824	0.7	0.822	0.5	0.819	0.1	0.818
		3070	0.903	0.3	0.902	0.2	0.901	0.1	0.901	0.1	0.900	0.0	0.900
	100% THF	46890	0.419	4.0	0.415	3.0	0.412	2.2	0.408	1.2	0.406	0.7	0.403
		20310	0.628	1.9	0.624	1.3	0.622	1.0	0.619	0.5	0.618	0.3	0.616
		7360	0.790	0.8	0.787	0.4	0.787	0.4	0.785	0.1	0.785	0.1	0.784
		3070	0.876	0.3	0.873	0.0	0.874	0.1	0.873	0.0	0.874	0.1	0.873