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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Dec 20;1864(3):358–371. doi: 10.1016/j.bbalip.2018.12.012

Fig. 2.

Fig. 2.

Hepatic ACSL1 deficiency induces hypercholesterolemia in mice fed a HFD. After 11-days of adenoviral injection, mice were fasted 4 h and then sacrificed for serum and liver sample collections. TC and FC were measured. CE and ester ratio were calculated as indicated in the Materials and methods section. In A–I, significance was determined by Student’s t-test. n = 6 mice per group. *p < 0.05, **p < 0.01, and ***p < 0.001.

(A) Serum TC.

(B) Serum LDL-C.

(C) Serum HDL-C.

(D) Serum FC.

(E) Calculated serum CE.

(F) Calculated serum CE ratio.

(G) Serum triglyceride.

(H) Serum free FA levels.

(I) Serum PL levels.

(J–K) Serum samples from two animals of the same treatment group were pooled together and a total of three pooled serum samples from each group were analyzed for cholesterol (J) and triglyceride (K) distribution in HPLC-separated lipoprotein factions. n = 3, *, p < 0.05.