| Component | Amount (μl) | Final concentration |
|---|---|---|
| DNA (Step 67) | 13 | — |
| 10× Ligation buffer (provided with T4 DNA ligase) | 2 | 1× |
| 2 μM adaptor | 1 | 0.1 μM |
| T4 DNA ligase | 4 | 80 U/μl |
| Total | 20 | — |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.