R-ChIP for genome-wide mapping of R-loops by using catalytically inactive RNASEH1

. Author manuscript; available in PMC: 2019 Jul 2.

Published in final edited form as: Nat Protoc. 2019 Apr 17;14(5):1661–1685. doi: 10.1038/s41596-019-0154-6

Step	Denature	Anneal	Extend	Hold
1	98 °C, 30 s
2 (14 or 16 cycles)	98 °C, 10 s	65 °C, 30 s	72 °C, 30 s
3			72 °C, 5 min
4				4 °C