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. Author manuscript; available in PMC: 2019 Jul 2.
Published in final edited form as: Nat Protoc. 2019 Apr 17;14(5):1661–1685. doi: 10.1038/s41596-019-0154-6
Component Amount (μl) Final concentration
DNA ligation product (Step 73) 16 —
5× Phusion HF buffer 20 1×
10 μM Barcode primer (Table 1) 2 0.2 μM
10 μM PCR primer (Table 1) 2 0.2 μM
10 mM dNTP mix 4 0.4 mM
Phusion High-Fidelity DNA polymerase 1 0.02 U/μl
Molecular-grade H2O 55
Total 100