Figure 1.

A Simplified Model of NFκB Activity (SiMoN) can predict NFκB activity from 3 parameters. (A) Schematic of the key reactions controlling NFκB activity through IκB metabolism. The amount of free, transcriptionally active, NFκB (NFκB activity) is tightly controlled by the amount of IκB; therefore IκB synthesis (reaction T) and free IκB degradation (reaction P) may potentially offer alternative points of control. The primary, canonical activation pathway is through IKK (reaction K), however, interferons do not directly activate IKK. (B) Schematic of the Simplified Model of NFκB (SiMoN), which analytically calculates NFκB as a result of parameters T,P and K. (C) Modeled time-course concentrations of free NFκB (lower), in response to perturbed reaction rates obtained by multiplying the WT parameter value by the multiplier indicated (upper) utilizing the simplified model. (D) Steady-state free NFκB concentrations in response to: increased IKK activity and IκB translation inhibition (left) and increased IKK activity and free IκB degradation (right).