Fig. 3.

Histological analysis of Huh7 xenograft tumours. Tumours were exposed to either no treatment (SHAM), or randomly chosen frequencies (RCF) or HCC-specific frequencies (HCCMF) 3 h daily at a SAR of 67 mW/kg. The selected control tumours (SHAM & RCF) grew in size while tumours exposed to HCCMF shrank by approximately 70%. a, Fibroblast-like cells intermeshed with tumour cells following HCCMF mediated tumour shrinkage. Histological analysis 10X (Lens; scale bar is 100um) shows residual tumour cells (➔) surrounded by layers of fibroblast-like cells (#) and occasional lipocytes. b, Epithelial neoplasm (Huh7-GFP tagged cells) intermeshed with vector red positive cells (➔) of spindle morphology demonstrating the same cell of origin for the two morphologically different populations 10× (Lens; scale bar is 100um). c, IHC summary of multiple IHC stains for SHAM, RCF, and HCCMF treated tumours. d, IHC staining images of Huh7 tumours following treatment. Images at 20× (Lens; scale bar is 50 um) with upper right embedded image at 60× (lens). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)