Fig. 2.

TRIM33 deficiency in mature myeloid cells modifies monocytes, macrophages and neutrophils numbers in colon of DSS-treated mice.

A. Percentages of Ly6C^high inflammatory blood monocytes (Cd11b⁺ CD115⁺ Ly6C^high) (left panel) and blood neutrophils (CD11b⁺ Ly6C^med Ly6G⁺) (right panel) without DSS treatment (d0) in WT (blue) and Trim33^−/− (red) mice. Mean ± SEM are performed with n = 30–40 mice per group. B–F. Myeloid sub-population during DSS-induced colitis. C–F. Of note the number of colonic cells did not change during DSS-induced colitis in WT and Trim33^−/− mice (Supplementary Fig. 2G) and thus percentages reflected the number of cells. B. Percentages of Ly6C^high inflammatory blood monocytes (left panels) and blood neutrophils (right panels) in WT (blue) and Trim33^−/− (red) mice. Mean ± SEM are performed with n = 30–40 mice per group. C. Percentage of id blood monocytes (CD45.2⁺ CD11b⁺ CMHII⁻ Ly6C^high) from colon of WT (blue) and Trim33^−/− (red) mice. Mean ± SEM are performed with n = 10–20 mice per group. D. Fold increase of Trim33^−/− over WT inflammatory blood monocytes (purple) and fold increase of Trim33^−/− over WT colonic id blood monocytes (pink) at day 16. Mean ± SEM are performed with n = 30–40 mice per group. E. Percentage of intermediate monocytes (CD45.2⁺ CD11b⁺ CMHII⁺ Ly6C⁺ F480⁻ CD64⁺) from colon of WT (blue) and Trim33^−/− (red) mice. Mean ± SEM are performed with n = 10–20 mice per group. F. Percentage of macrophages (CD45.2⁺ CD11b⁺ CMHII⁺ Ly6C⁻ F480⁺ CD64⁺) from colon of WT (blue) and Trim33^−/− (red) mice. Mean ± SEM are performed with n = 10–20 mice per group. G. Percentage of neutrophils (CD45.2⁺ CD11b^high CMHII⁻ Ly6C^med Ly6G⁺) from colon of WT (blue) and Trim33^−/− (red) mice. Mean ± SEM are performed with n = 6–20 mice per group. Statistical comparisons were performed with Mann-Whitney test (*p < 0.02; **p < 0.0003; ***p < 0.0006; ****p < 0.0001).