Fig. 4.

Defects of Trim33^−/− colonic macrophages during resolution of colitis.

A. Percentages of WT (blue) and Trim33^−/− (red) CD206⁺ M2 colonic macrophages during DSS-induced colitis (left panel). Mean ± SEM are performed with n = 6–9 mice per group. mRNA level of Retnla in WT (blue) and Trim33^−/− (red) colonic macrophages at day 29 (right panel). Mean ± SEM are performed with n = 3 mice in each group. qRT-PCR data are normalized to Hprt. B. mRNA level of Tnf in WT (blue) and Trim33^−/− (red) colonic macrophages at day 29. Mean ± SEM are performed with n = 3 mice in each group. qRT-PCR data are normalized to Hprt. C. Percentage of colonic macrophages expressing TNF at day 29 in WT (blue) and Trim33^−/− (red) mice. Mean ± SEM are performed with n = 6 mice in each group. D. Percentage of colonic macrophages expressing membrane-bound TNF (mTNF) at day 29 in WT (blue) and Trim33^−/− (red) mice (left panel). Mean of fluorescence (MFI) of mTNF in WT (blue) and Trim33^−/− (red) colonic macrophages at day 29 (right panel). Mean ± SEM are performed with n = 6 mice in each group. Statistical comparisons were performed with either a Student's t-test or a Mann-Whitney test as appropriate (*p < 0.05; **p < 0.003).