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. Author manuscript; available in PMC: 2019 Nov 14.
Published in final edited form as: Sci Signal. 2019 May 14;12(581):eaau0615. doi: 10.1126/scisignal.aau0615

Figure 2. BCAP interacts with NLRP3 inflammasome components through its N-terminal domain.

Figure 2.

(A and B) Western blot analysis of the indicated proteins in lysates from 293T cells co-transfected with plasmids encoding BCAP-Flag, Flightless-1, LRRFIP2-HA, NLRP3, ASC, and pro-caspase-1-RFP as indicated and immunoprecipitated for Flag. Black arrowhead in (B) indicates LRRFIP2 band. (C) Flag-tagged BCAP deletion mutants used in (D). (D) Western blot analysis of 293T cells co-transfected with plasmids encoding BCAP-Flag deletion mutants and Flightless-1. Lysates were immunoprecipitated for Flag and immunoblotted with anti-Flag and anti-Flightless-1 antibodies. Data are representative of 3 independent experiments.