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. Author manuscript; available in PMC: 2019 Nov 14.
Published in final edited form as: Sci Signal. 2019 May 14;12(581):eaau0615. doi: 10.1126/scisignal.aau0615

Figure 5. BCAP inhibits NLRC4 inflammasomes.

Figure 5.

(A) Immunofluorescence staining for active caspase-1 (left) and LDH assay of cell death (right) in unprimed BMDMs infected with S. Typhimurium for 30 minutes. Data are means + SEM pooled from 3 independent experiments. (B) Western blot analysis of pro-caspase-1 cleavage in lysates of BMDMs infected with S. Typhimurium for 30 minutes. Blots (left) are representative of 3 independent experiments and quantified band intensity values are means + SEM pooled from all experiments. (C) ELISA analysis of IL-1β release from LPS-primed BMDMs infected with S. Typhimurium for 30 minutes. Data are means + SEM pooled from 3 independent experiments. (D) LDH assay of cell death in unprimed BMDMs treated with FlaTox for 20 minutes. Data are individual means from 5 independent experiments (connected with lines). (E) Live cell imaging to measure cell death in BMDMs treated with RodTox. Data are means ± SEM of 3 independent experiments. (F) LDH assay of cell death in unprimed BMDMs transfected with calf thymus DNA for 3 hours. Data are means + SEM pooled from 3 independent experiments. (G) LDH assay of cell death in unprimed BMDMs treated with LPS and Cholera Toxin B for 3.5 hours. Data are means ± SEM of 3 independent experiments. *p<0.05, **p <0.01, ***p<0.001 by two-sided unpaired (A-C and E-G) and paired t-test (D).