Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
18:2 synthesis in yeast expressing wild-type or mutant Arabidopsis FAD2 proteins
| Strain | Mutation | 18:2 synthesis | |
|---|---|---|---|
| 30°C | 20°C | ||
| Empty vector | 0 | 0 | |
| WT | – | 32.1 ± 4.5 | 58.5 ± 1.5 |
| fad2-1 | A104T | 2.1 ± 0.1 | 40.3 ± 1.3 |
| fad2-2 | S136F | 0 | 0 |
| fad2-3 | W287stop | 0 | 0 |
| fad2-4 | P280L | 0 | 0 |
| fad 2-6 | T148I | 6.1 ± 1.0 | 35.1 ± 0.8 |
Yeast expressing each protein were cultured for 48 h at either 30 or 20°C.
Data are 18:2 as percentage of 18:1 and 18:2; mean ± SE from three separate experiments.
WT, wild-type.
