Table 3.
18:2 synthesis in yeast expressing wild-type or mutant Brassica FAD2.A5 proteins
| Mutation | 18:2 synthesis | |
|---|---|---|
| 30°C | 20°C | |
| Empty | 0 | 0 |
| vector | ||
| WT | 66.6 ± 0.4 | 67.8 ± 1.3 |
| G257R | 0 | 0 |
| P159L | 0 | 0 |
| G123D | 0 | 0 |
| E152K | 0 | 0 |
| T297I | 0 | 0 |
| S199L | 0.19 ± 0.15 | 1.84 ± 0.78 |
| G200R | 3.22 ± 0.13 | 8.68 ± 0.54 |
| P216L | 3.57 ± 0.27 | 21.3 ± 3.9 |
| G232D | 5.11 ± 0.69 | 33.1 ± 1.1 |
| S150F | 8.56 ± 0.50 | 40.4 ± 5.4 |
| G248R | 20.7 ± 1.5 | 64.9 ± 1.3 |
| P191L | 24.3 ± 2.4 | 54.5 ± 2.0 |
| G232S | 36.8 ± 6.3 | 65.9 ± 1.7 |
| P202L | 40.7 ± 2.6 | 64.0 ± 3.4 |
| E287K | 57.7 ± 1.7 | 76.9 ± 2.6 |
Yeast expressing each protein was cultured for 48 h at either 30 or 20°C.
Data are 18:2 as percentage of 18:1 + 18:2; mean ± SE for three separate experiments.
WT, wild-type.