Figure 2.

γ-Secretase activity of single-Cys mt PS1 used in this study. A, Sandwich ELISA of secreted Aβ from APPNL-stable DKO cells transiently transfected with single-Cys mt PS1 (n = 3–12, means ± SEs). The levels of secreted Aβ in the conditioned media were normalized by that of cells transfected with PS1/Cys(−). Amounts of Aβ₄₀ production are shown as white bars, and amounts of Aβ₄₂ production are shown as black bars. B, NICD generation from NotchΔE-stable DKO cells transiently transfected with single-Cys mt PS1 was detected by immunoblot analysis using an anti-cleaved Notch1 antibody (top panels). The levels of expressed PS1 NTF and Pen-2 were detected by immunoblot analysis using anti-PS1 NTF and Pen-2 antibodies (bottom panels).