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. 2016 Jan 27;36(4):1203–1210. doi: 10.1523/JNEUROSCI.0436-15.2016

Figure 3.

Figure 3.

Panx1 is essential for maintaining elevated VZ NPC numbers after stroke. Ai, Panx1-null and Panx1-expressing NPC numbers per VZ at 2, 5, and 10 dpi/PT. Naive/sham data from Figure 2A are overlaid in light gray. The number of Panx1-null NPCs significantly decreased over time (2 dpi/PT, n = 7; 5 and 10 dpi/PT, n = 6, expression: F(1,32) = 2.854, p = 0.1008; time: F(2,32) = 4.644, p = 0.0169; interaction: F(2,32) = 4.902, p = 0.0139 by 2-factor ANOVA; Bonferroni post hoc *p < 0.05 for expression at 10 dpi/PT). Aii, Maximum-intensity projections of representative confocal Z-stacks of the VZ show transduced NPCs at 2 (left) and 10 dpi/PT (right). Scale bars, 10 μm. V, Ventricle; cc, corpus callosum; str, striatum. Hoechst 33342 was used as a nuclear counterstain. Bi, The vast majority of transduced VZ NPCs were immunoreactive for DCX (2 dpi/PT, n = 6, p = 0.5202 by unpaired t test). Bii, Maximum-intensity projection of a representative confocal Z-stack from the VZ demonstrating DCX-immunoreactivity of transduced VZ NPCs. Scale bar, 10 μm. Arrows indicate DCX+ transduced NPCs. Ci, The percentage of transduced VZ NPCs immunoreactive for Ki67 was not affected by Panx1 expression status, but was affected by time after stroke (2 dpi/PT, n = 7, 5 and 10 dpi/PT, n = 5; expression: F(1,28) = 0.8049, p = 0.3760; time: F(2,28) = 3.461, *p = 0.0454; interaction: F(2,28) = 0.1508, p = 0.8607 by 2-factor ANOVA). Cii, Maximum-intensity projection of a representative confocal Z-stack demonstrating Ki67 immunoreactivity of transduced VZ NPCs. Scale bar, 10 μm. Arrows indicate Ki67+-transduced NPCs. Hoechst 33342 was used as a nuclear counterstain. D, Quantification of Panx1-null and Panx1-expressing NPC numbers in the RMS at 2, 5, and 10 dpi/PT. The number of transduced NPCs was not affected by Panx1 expression status and did not significantly change over time (2 and 5 dpi/PT, n = 6; 10 dpi/PT, n = 5; expression: F(1,28) = 3.593, p = 0.0684; time: F(2,28) = 1.812, p = 0.1820; interaction: F(2,28) = 1.998, p = 0.1545 by 2-factor ANOVA).