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. 2016 Jan 27;36(4):1151–1164. doi: 10.1523/JNEUROSCI.1797-15.2016

Figure 5.

Figure 5.

Tango1 mediates laminin secretion via a collagen-independent mechanism. A, Low-magnification image of control ventral nerve cord and peripheral nerves in larvae with 46F-GAL4 driving mCD8::RFP in perineurial glia (red), and collagen-IV endogenously tagged with GFP and Viking::GFP (green), and immunolabeled for LanB2 (blue). Scale bar, 100 μm. B, High-magnification image of nerve section in A. Scale bar, 15 μm. Digitally magnified section of ECM in inset shows two distinct layers of LanB2. Viking::GFP is not observed in the interior of the nerve. C, Low-magnification image of ventral nerve cord and peripheral nerves in 46F>Tango1-RNAi larvae with the perineurial glia membrane labeled with mCD8::RFP (red) and Viking::GFP (green), and immunolabeled for LanB2 (blue). Loss of Tango1 led to accumulations of LanB2 (yellow arrows) but not Viking::GFP. Scale bar, 100 μm. D, High-magnification image of a nerve in C. Scale bar, 15 μm. Digitally magnified section of ECM in inset shows one layer of LanB2. Viking::GFP was not observed in the interior of the nerve, while LanB2 was observed in intracellular accumulations (yellow arrows).