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Observation of w-ions in ECD enable differentiation of Ile and Leu residues. Zoomed in portions of an ECD spectrum of the 10+ charge state of ubiquitin showing product ions that confirm L73 (A) and I61 (B). The spectrum was acquired with 50 microscans and a resolving power of 120 000 at m/z 200. S/N for the w4-and w163+-ions are 34 and 12, respectively.
