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. Author manuscript; available in PMC: 2019 Jul 2.
Published in final edited form as: Calcif Tissue Int. 2017 Aug;101(2):204–206. doi: 10.1007/s00223-017-0281-4

Fig. 5.

Fig. 5

(Top) Representative confocal microscopy images demonstrating the presence of fluorochrome labels in the endocortical and intra-cortical compartments of both virgin/normal Ca and lactation/ low-Ca groups. The virgin/normal Ca controls lacked fluorochrome labelsin the periosteal compartment. Oxytetracycline (labeled ‘T’ in the images) was given on day 2; calcein (‘C’) was given on day 14; xylenol orange (‘X’) was given on day 21; and alizarin (‘A’) was given on day 26 of the recovery phase. (Bottom) Degree of mineralization (mineral-to-matrix ratio) plotted as a function of tissue age and compartment. Data are presented as the means and standard deviations. There was not a significant difference between groups in the intra-cortical compartment. Endocortically, the two-way ANOVA indicated a significant tissue age effect (p = 0.031) and a significant interaction term (p = 0.013), with no significant group effect (p =0.602)