Figure 3.

Decreased survival rate of Syt10 neurons in response to KA can be rescued by a secreted factor. A, Syt10 KO neurons were incubated at 13–14 DIV for 24 h with or without 25 μm KA and in the absence or presence of medium from WT neurons. Subsequently, cells were immunolabeled for MAP2, GFAP, and DAPI and neuronal survival was quantified by normalizing the number of MAP2-positive cells to the total cell number represented by DAPI in staining. Statistical analysis was performed using one-way ANOVA followed by Tukey post hoc test. **p ≤ 0.01, ***p ≤ 0.001, N = 5, n = 3–4. B, To assess rescue efficiency the number of surviving neurons after KA incubation was normalized to the rescue condition. C, Neurons were incubated at 14 DIV for 24 h with or without 25 μm KA and IGF-1 and the level of neuronal cell death was analyzed as described in A. Statistical analysis was performed using one-way ANOVA followed by Tukey post hoc test. ***p ≤ 0.001; N = 2, n = 4.