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. 2016 Mar 2;36(9):2561–2570. doi: 10.1523/JNEUROSCI.2027-15.2016

Figure 6.

Figure 6.

NPAS4 elevates Syt10 expression in vitro and in vivo. A, B, Quantitative RT-PCR on mRNA extracted from RH neurons infected with viral particles at 7 DIV encoding a rAAV-NPAS4 overexpression construct (NPAS4). Neurons were lysed at 14 DIV and mRNA expression levels of NPAS4 (A) and Syt10 (B) were measured (N = 3, n = 3). C, D, Quantitative RT-PCR on mRNA extracted from the hippocampal subregion CA1 from control (rAAV-GFP; n = 7) and rAAV-NPAS4-injected (n = 7) mice (N = 1). E, F, Quantitative RT-PCR analysis for NPAS4 (E) and Syt10 (F) on RH neurons (14 DIV) transduced at 9 DIV with either a scrambled shRNA (control shRNA) or an shRNA directed against NPAS4; N = 5, n = 3. Statistical analysis was performed using Student's t test. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.