Figure 7.

NPAS4 rescues the KA-induced cell death only in Syt10 WT neurons. A, B, Dissociated Syt10 WT (A) and KO (B) neurons were transduced at 9 DIV with a control virus (rAAV-GFP) or with a virus encoding a rAAV-NPAS4 overexpression construct (NPAS4). At 13 DIV, neurons were stimulated, stained, and cells that were positive for MAP2 were counted with and normalized to the total cell number (DAPI). All values were normalized to the GFP basal values. C, Difference of the NPAS4-mediated rescue of kainate-treated Syt10 WT compared with Syt10 KO neurons. All values were normalized to the WT values. Statistical analysis was performed using a one-way ANOVA followed by Tukey post hoc test (n.s. > 0.05, ***p ≤ 0.001; A, B) or using Student's t test (***p ≤ 0.001; N = 1, n = 3; C).