Figure 4.
Forebrain cholinergic tone regulates translation of hNRNPA2/B1 in the hippocampus. A, Hippocampal brain tissue was fractionated on a linear sucrose gradient. Fractions were collected and analyzed by spectrophotometry to determine position of the monosome (80s), polysomes, and RNA granules. B, RT-PCR of hnRNPA2/B1 transcripts in VAChTflox/flox and VAChTNkx2.1-Cre-flox/flox mice in the absence or presence of EDTA. C, RT-PCR of β-actin transcripts in VAChTflox/flox and VAChTNkx2.1-Cre-flox/flox mice in the absence or presence of EDTA. D, E, Quantification of data for the hnRNPA2/B1 transcripts from the three different VAChT-deficient and three control mice in the absence or presence of EDTA. F, G, Quantification results for β-actin transcripts from the three different VAChT-deficient and three control mice in the absence or presence of EDTA. The numbers 1–12 are the fraction numbers. The values plotted are averaged from gels in C. Data shown are means.