Table 1.
Antibodies and methods of detection
| Marker | Primary antibody | Secondary antibody | Technique/coloration |
|---|---|---|---|
| BrdU | Mouse, 1:100, Dako | Horse anti-mouse, 1:200, Vector Laboratories | DAB/brown |
| GFAP | Rabbit, 1:200, Dako | Goat anti-rabbit, 1:200, Vector Laboratories | DAB/brown |
| GAD67 | Mouse, 1:1000, Chemicon | Horse anti-mouse, 1:200, Vector Laboratories | DAB/brown |
| BrdU | Rat, 1:100, Oxford Biotech | Rabbit anti-rat, 1:200, Interchim | Alexa Fluor 594/red |
| GFAP | Rabbit, 1:200, Dako | Goat anti-rabbit, 1:200, Interchim | Alexa Fluor 488/green |
| GAD67 | Mouse, 1:100, Chemicon | Rabbit anti-mouse, 1:200, Interchim | Alexa Fluor 488/green |
| NeuN | Mouse, 1:100, Chemicon | Rabbit anti-mouse, 1:200, Interchim | Alexa Fluor 488/green |
| IBA1 | Rabbit, 1:2000, Wako | Goat anti-rabbit, 1:200, Interchim | Alexa Fluor 488/green |
| ChAT | Goat, 1:5000, Chemicon | Donkey anti-goat, 1:2000, Jackson ImmunoResearch | DAB/blue |
| KCC2 | Rabbit, 1:250, Millipore | Donkey anti-rabbit, 1:500, Jackson ImmunoResearch | Cy3/red |
| GABAA | Mouse, 1:100, US Biol Life | Donkey anti-mouse, 1:800, Life Technologies | Alexa Fluor 488/green |
Shown are the combination and sequential processing of primary and secondary antibodies used for immunohistochemical and dual immunofluorescent stainings for BrdU, NeuN, GAD67, ChAT, GABAA receptor, KCC2, GFAP, or IBA1.