Figure 7.

Immunohistochemistry for BBB integrity. A, Lumbar spinal cord sections from a bronchial asthma model mouse stained with anti-intercellular adhesion molecule-1 (ICAM-1) antibody. Scale bar, 100 μm. B, Lumbar spinal cord sections from a bronchial asthma model mouse double-stained with anti-mouse albumin and anti-GFAP antibodies. Scale bar, 50 μm. In an O+A-pretreated mouse with asthma, endothelial cells in the spinal cord become ICAM-1-positive (white arrowheads), and albumin leaks out of the vessels into the spinal cord parenchyma (white arrowheads).