Figure 1.

Characterization of hiPSC lines. A, All hiPSC lines used in this study expressed the pluripotency markers NANOG, OCT4, TRA1–81, and SSEA4 as seen by immunocytochemical stainings. B, To show pluripotency, iPSC lines were differentiated in vitro into cells expressing markers of all three germ layers: ectoderm (TUBB3), endoderm (AFP), and mesoderm (SMA). For A and B, WT1–1 and Mut1 are shown as representative lines. Nuclei were counterstained with Hoechst. Scale bars, 100 μm. C, qRT-PCR revealed that all iPSC lines expressed a comparable level of pluripotency markers as the hESC line HUES6. D, Retroviral transgenes used for reprogramming were efficiently silenced in established iPSC lines. Expression was normalized to a freshly infected human fibroblast sample 4 d postinfection (dpi). Data are presented as means ± SEM using GAPDH and BACT as housekeeping genes.