Figure 2.

Sequential induction of hiPSCs toward MSNs. A, Schematic summary of the differentiation procedure. B–D, Phase-contrast images during in vitro differentiation. hiPSC colonies were detached from the feeder layer and cultured in suspension as EBs in the presence of small molecules as indicated. On days 12–14, EBs were plated on Matrigel-coated cell culture dishes. Neuronal cells started to spread out and matured through the addition of various growth factors. Scale bars, 100 μm.