Figure 6.

Elevated AP amplitudes in ChAc neurons. A, Differentiated cells from ChAc patients and healthy controls fired single APs in response to depolarizing current pulses in current-clamp mode that could be blocked by 1 μm TTX. B, Although the percentage of cells with APs did not differ in either group, the AP amplitudes were significantly higher in ChAc neurons compared with controls. C, Spontaneous AP firing was measured at the neuronal membrane potential over a period of 11 s. D, Spontaneous APs were observed only in a minor cell population without significant differences in percentage or frequency, but higher amplitudes in ChAc neurons compared with controls. Data represent means ± SEM (p < 0.05, Mann–Whitney test).