Table 2.
Functional properties of neurons from ChAc-iPSCs and healthy controls
| Functional properties | Mut (n = 141) | WT (n = 150) | p-values |
|---|---|---|---|
| Cells with Na+-currents (%) | 88.6 ± 4.8 | 77.4 ± 7.1 | 0.2415 |
| Peak Na+-current (pA/pF) | −124.0 ± 8.9 | −104.7 ± 7.9 | 0.0930 |
| Peak K+-current (pA/pF) | 126.6 ± 8.0 | 110.1 ± 7.3 | 0.0508 |
| Na+ channel activation (mV)*** | −28.2 ± 0.7 | −22.4 ± 0.8 | <0.0001 |
| K+ channel activation (mV)*** | −20.3 ± 0.9 | −15.3 ± 0.9 | 0.0001 |
| Resting membrane potential (mV) | −35.3 ± 0.9 | −32.3 ± 1.2 | 0.1568 |
| Membrane capacitance (pF)*** | 14.9 ± 0.6 | 12.5 ± 0.7 | 0.0006 |
| Input Resistance (MOhm) | 1558.0 ± 111.2 | 1389.0 ± 90.9 | 0.4835 |
| Cells with APs (%) | 58.7 ± 10.3 | 45.7 ± 9.6 | 0.3257 |
| AP amplitude (mV)** | 49.9 ± 1.7 | 42.2 ± 2.0 | 0.0041 |
| AP duration (msec)*** | 2.2 ± 0.1 | 3.1 ± 0.2 | <0.0001 |
| AHP amplitude (mV)** | 19.3 ± 0.7 | 16.5 ± 0.8 | 0.0093 |
| Time to peak AHP (msec) | 7.6 ± 0.2 | 8.1 ± 0.3 | 0.4117 |
| Cells with spontaneous APs (%) | 13.9 ± 3.8 | 6.7 ± 2.4 | 0.1220 |
| Amplitude of spont. APs (mV)** | 39.1 ± 0.7 | 34.6 ± 0.6 | 0.0012 |
| Frequency of spont. APs (Hz) | 2.4 ± 0.5 | 2.0 ± 0.8 | 0.4005 |
| Cells with mPSCs (%)** | 88.6 ± 4.3 | 69.5 ± 5.3 | 0.0087 |
| mPSC amplitude (pA)*** | 11.7 ± 0.1 | 9.5 ± 0.4 | <0.0001 |
| mPSC frequency (Hz)*** | 0.57 ± 0.06 | 0.30 ± 0.03 | <0.0001 |
Voltage-gated ion channel, passive membrane, and AP properties were determined by whole-cell patch-clamp recordings. Data are given as means ± SEM. AHP, Afterhyperpolarization.