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Wild type MG1655 and the isogenic gadC and yhiM mutants were tested for different AR phenotypes. G glutamate- and glutamine-dependent AR were assayed by diluting 1:1000 24-hours cultures grown in LBG pH 5.0 in the indicated media. The oxidative AR system activity was assayed by diluting 1:1000 24-hours cultures grown in LB-MES pH 5.5 in the indicated media.
