Table 1. Bacterial strains and plasmids used in this study.
| Strain or Plasmid | Relevant Genotype | Reference or source |
| MC4100 | F− araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC ptsF25 rbsR | [34] |
| MG1655 | F− λ− rph−1 | GSC |
| JM109 | (F' traD36 proA+ proB+ lacIq lacZΔM15) recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB) | [31] |
| MC4100gadX | MC4100 gadX::KanR | [9] |
| MC4100gadE | MC4100 gadE::KanR | [35] |
| MG1655gadX | MG1655 gadX::KanR | [9] |
| MG1655gadE | MG1655 gadE::KanR | [35] |
| MG1655yhiM | MG1655 yhiM::KanR | This work |
| MC4100yhiM | MC4100 yhiM::KanR | This work |
| MG1655gadC | MG1655 gadC::KanR | [36] |
| MC4100gadC | MC4100 gadC::KanR | This work |
| pBs | (pBluescriptSK) multicopy phagemid vector; ColE1 replicon, lacZ bla | Stratagene |
| pCRIIyhiM | 1050-bp fragment encompassing the entire yhiM coding sequence ligated in pCRII-TOPO | This work |
| pQE60 | Expression vector; ColE1 replicon, PT5-lacO RBSII | QIAGEN |
| pQEyhiM-6×His | 1050-bp fragment encompassing the entire yhiM coding sequence ligated to the NcoI-BglII sites of pQE60 | This work |