Table 2. Oligonucleotides used in this study.

Primer	Sequence		Restriction site
ΔyhiM_for	5′-ATTATCATTAATGCATATTTCAATATTAGCAGGGATACC gtgtaggctggagctgcttc-3′
ΔyhiM_rev	5′-CCGCTTCTAATATTGAAACGATTGAGAACAACGTAAAGC attccggggatccgtcgacc-3′
ΔgadC_for	5′-TACCGTTTTAGGGGGATAATATGGCTACATCAG TACAGACgtgtaggctggagctgcttc-3′
ΔgadC_rev	5′-TTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAA attccggggatccgtcgacc-3′
yhiM_revRT	5′-TAACAGTGCCCAACCCCATATCAT-3′
yhiM_for	5′-ggataccatgGTGAACATATATATCGGGTGG-3′		NcoI
yhiM (Bgl)_rev	5′-ggttagatctTTTTTTAGCAGAACCCGCTTC-3′		BglII
gadC_rev	5′-GCACATATGGATATCTGCTCCC-3′
α/β_out	5′-CGAAACTGCAGGGTATTGC-3′
rRNA16S_for	5′-CGTTACCCGCAGAAGAAGC-3′
rRNA16S_rev	5′-GTGGACTACCAGGGTATCTAATCC-3′
k1	5′-CAGTCATAGCCGAATAGCCT-3′

Restriction sites are underlined. Nucleotides not matching the target sequence are in lower-case.