Table 4. Partition of Glutamate decarboxylase (GadA/B) activity* in the cytosolic (Cyto) and membrane (Mem) fractions from stationary phase cellular extract brought to pH 7.4 or 5.6.
pH 7.4 | pH 5.6 | |||
Cyto |
Mem |
Cyto |
Mem |
|
MC4100 | 265 ± 21 | 75 ± 6 | 146 ± 11 | 124 ± 9 |
MC4100ΔyhiM | 275 ± 23 | 56 ± 4 | 190 ± 8 | 143 ± 5 |
* the activity is reported in Units/mg.