Figure 2. Real time profile of lab scale fed-batch fermentation process for the expression of recombinant dengue virus type 2 DIII protein. Cultivation was carried out in modified SB medium and culture was induced with IPTG (1 mM) at DCW of ∼16 g/l for rDen2 DIII protein expression. Figure shows the time profile for agitation (RPM), pH, temperature (°C), DO concentration (%), air flow rate (liter per minute), and OD600.
