Figure 5. Chromatogram showing pilot scale purification of rDen2 DIII protein using Immobilized metal affinity affinity chromatography. The solubilized IBs containing rDen2 DIII protein was applied onto a pilot scale chromatography column (2 liter capacity Quik Scale column) containing 500 ml charged streamline chelating affinity chromatography resin. Unbound proteins were removed by washing the column with wash buffer (ten column volume) and purified protein was eluted with elution buffer.
