Figure 6. Characterization of pilot scale purified rDen2 DIII protein produced at 100 liter scale. (a) SDS-PAGE (Coomassie stained) profile of rDen2 DIII protein. Lane 1, MW Marker (kDa); Lane 2, Un-induced culture; lane 3, Lysate; lane 4, Load; Lane 5, Purified protein. (b) Purity of rDen2 DIII protein was assessed on silver stained SDS-PAGE gel. Lane 6, Molecular Weight Marker (kDa); Lane 7, Un-induced culture; lane 8, Lysate; lane 9, Load; lane 10, Purified protein.