Table 1. Production characteristics of rDen 1 DIII protein using modified SB medium in E. coli.
Cultivation mode | Dry cell weight (g/l) | Total protein concentration (g/l) | Final product concentration (mg/l) | Specific product yield (mg/g) |
Shake flask | 2.56 | 0.657 | 16 | 6.26 |
Batch fermentationa | 5.0 | 1.34 | 62 | 12.40 |
Fed-batch fermentationb | 16.0 | 3.38 | 161 | 10.06 |
aAt 5 liter scale; bAt 10 liter scale.