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. Author manuscript; available in PMC: 2019 Jul 2.
Published in final edited form as: Methods Mol Biol. 2019;1919:161–173. doi: 10.1007/978-1-4939-9007-8_12

Fig. 1.

Fig. 1

Workflow for screening mitoeffective compounds using iPSC-derived dopaminergic neurons and XFe96 analyzer. Generate iPSCs from patient somatic cells and isolate NSCs. Culture and differentiate the NSCs into dopaminergic neurons in cell culture plate of XFe96 analyzer. Treat (acute or chronic) the dopaminergic neurons with small molecule library. Measure changes in OCR using XFe96 analyzer. Evaluate the effect of the test compounds on different components of mitochondrial respiration. Select potential compounds for further testing from the pool based on their efficacy on improving mitochondrial respiration