Skip to main content
. Author manuscript; available in PMC: 2019 Jul 2.
Published in final edited form as: J Vis Exp. 2019 Feb 28;(144):10.3791/59015. doi: 10.3791/59015

Figure 1: Correlation between OD600 and CFU counts for ALC2906.

Figure 1:

3–4 ALC2906 SH1000 colonies were picked from an agar plate and transferred into TSB with 10 μg/mL chloramphenicol for overnight culture. The next day, the suspension was split 1:50 into TSB with 10 μg/mL chloramphenicol and cultured. Optical density at 600 nm (OD600) was measured in regular intervals after 2 hours using a spectrophotometer.At each measurement the bacteria was diluted 1:100,000 in ice cold PBS and aliquoted onto an agar plate for overnight incubation. CFUs were counted the following day to calculate the initial concentration and correlated to OD600. N = 3 with 4 different OD600 measurements per experiment.