Figure 4.

Inhibition of endogenously active β2-nAChRs and α7-nAChRs enhances Up state activity. A, B, Up state duration and occurrence, respectively, are increased in the presence of the β2-nAChR antagonist DHβE (3 μm) in WT mice only (mean ± SEM, data normalized to values obtained in the absence of DHβE). C, D, The combined effect of β2 and α7 inhibition (3 μm DHβE and 10 nm MLA) is an increase of both duration and occurrence in WT animals and an increase in duration in β2 mutants (mean ± SEM, data are normalized to the values obtained in the absence of any drug). E, F, The α7-nAChRs antagonist MLA (10 nm) causes an additional increase of Up state duration in both WT and β2^−/− mice, over and above the effect of DHβE, but has no significant effect on Up state occurrence (mean ± SEM, data normalized to values obtained in the presence of DHβE). G, H, Non-normalized values of Up state duration and occurrence, respectively, before and after the addition of DHβE and MLA (mean ± SEM). Black and gray lines indicate WT and β2^−/− mice, respectively. Data shown are pooled from both age groups (three-way repeated-measures ANOVA, with pairwise comparisons post hoc tests, *p < 0.05, **p < 0.01, and ***p < 0.001).